Hipure Ffpe Dna Column - China

The HiPure FFPE DNA Column provides a simple and reliable method for high yield/quality DNA isolation from formalin-fixed, paraffin embedded (FFPE) tissue samples and sections.

The unique chemistries of the product have been optimized for maximum recovery of non-crosslinked, ultra-pure DNA without RNA contamination.

Simply digest deparaffinized tissues using Proteinase K, heat, and then purify the DNA with the FFPE DNA Column.

DNA >50 bp or >500 bp can be selectively isolated by altering the lysis buffer conditions with protocol.

PCR inhibitors are effectively removed during the isolation procedure, and eluted DNA is ideal for PCR, Next-Gen library prep, enzymatic manipulation, etc.

DNA Recovery High quality total DNA (A260/A280 >1.8) can be eluted into small volumes (i.e., ≥25 µl) allowing for highly concentrated samples.

The maximum DNA binding capacity of the provided spin column is ~25 µg

Processing Time As little as 4 hours when processing large amounts of tissue.

For maximum yields of the highest quality DNA, it is recommended to process samples overnight.

Equipment Microcentrifuge, thermomixer or heat block/bath capable of 55°C and 90°C, isopropanol, beta-mercaptoethanol (optional).

Sample Size Up to 25 mg tissue from paraffin block or up to four (4) tissue sections (≤20 µm thick) with a total surface area ~20 cm2 .

It is recommended to use 1-2 sections if performing the protocol for the first time.

Compatible with fresh/frozen tissue specimens

Company Contact:

• Posted By: Angen Biotech Co.,Ltd

• Phone: 02038555004

• Address: 8 Yayingshi Road, Luogang District, Guangzhou , Guangdong , China

• Email:

• Website: https://www.angenbio.com

• Business Description:
  
  ANGEN, a leading biotechnology company in China, provides professional biology products and technical service to customers in life science research field.
  
  In 2011, the company was founded by three scientists with a mission to produce innovative and cost-effective products for life science research.
  
  AnGen Biotech's headquarter, R&D, and manufacturing facility are located in Guanghzou.
  
  Now, the comany has more than 10 biologists and 15 sales officer in China.
  
  The comany has about 20 distributors in different cities in local market.
  
  And also have more than 35 customers oversea maily in Noth America, South America, Europe and Asia.
  
  We are targetting to help molecular companies which supplying DNA/RNA prufication reagents.
  
  AnGen Biotech was a professional company in producing Nucleic Purification Accessories like Plasmid DNA Extraction Mini Spin Columns, Genomic DNA Extraction Columns, Gel Purification Columns, RNA Extraction Columns, Maxi Spin Columns.
  
  And also Plastic materies like PCR Tubes, Centrifuge Tubes, Plastic Bottles and Storage Vials.